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In addition, the heat map showed that the logFC value of cell cycle related genes between PRR11 high and low groups. It was found that PTTG1 involved in the cell cycle was upregulated in 10 out of 11 cancers and downregulated in NCI-H460 and BxPC3 cancer cell lines with interfering PRR11 (Figure 5B). In addition, the rescue assay verified PRR11/PTTG1 promoting cell proliferation in NCI-H460 (Supplementary Figure S4B).

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The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. This section collects any data citations, data availability statements, or supplementary materials included in this article. MCF7 LTED (5 × 104/well) cells were seeded in 8-well chamber slides (Lab-Tek, ) in triplicate. HEK293 cells (5 × 104) were seeded 24 h post-transfection with pLX302-PRR11-V5, pLenti7.3-PIK3R1-Flag, and pLenti7.3-PIK3R1-HA. P85 (Cat# , Millipore), V5 (Cat# 13202, Cell Signaling Technology), HA (Cat# 3724, Cell Signaling Technology) and Flag (Cat# 8146, Cell Signaling Technology) antibodies were used for this assay.

We show herein that the PR domain of PRR11 physically binds the SH3 domain of p85, thus inhibiting p85 homodimer formation. Homodimers or monomers of p85 form a sequestration complex with IRS1, thus competing with p85-p110 dimers for binding to IRS1 at the plasma membrane and attenuating insulin/IGF stimulated PI3K activity39. Hence, an excess of PRR11 would favor p85-p110 dimers and be permissive of p110 binding to IRS1, retention of p110 at the plasma membrane, and enhanced PIP3 formation. Homodimers of p85 can also inhibit PI3K signaling via direct association and stabilization of PTEN37, but we did not observe that knockdown of PRR11 alters PTEN protein levels (Fig. 5a). Sports fans in Bangladesh can place bets on a wide range of sporting events.

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Spearman correlation between PRR11 and PTTG1

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The number of permutations was set at 1,000, and the cutoff P-adjust was 0.05 to filter the significant enrichment results. The protein–protein interaction (PPI) network of DEGs was obtained from the STRING (version 11.0) database (Szklarczyk et al., 2019). After 48 h from transfection with control siRNA or PRR11 siRNAs, cells were trypsinized and then fixed with 70% ethanol for 3 h at −20 °C. Cells were washed x3 with PBS and then resuspended in PBS containing 100 µg/mL RNase A and 40 µg/mL propidium iodide for 10 min at room temperature.

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And then, of course, there’s this friendship and here they are, and I look at this is my wife and her sister, is their siblings, you know, and my wife has no closer friend on the planet than her sister. So think of if you’ve ever had that just wonderful, beautiful, close, trusting friendship, how it can be a real mental stimuli, you know, it can be another of the same gender or someone of another gender. Now, remember that the brain is the 12th principle, and in this philosophy, we’re coming to that in another a couple of weeks. And what the brain is is a transmitting and sending unit for vibration energy, alright?

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